Vol. 12 No. 4 (2024): October-December issue

ANTIBIOTIC RESISTANCE PATTERNS OF COMMON UROPATHOGENS ISOLATED FROM FEMALES AT ZAKHO CITY, KURDISTAN REGION, IRAQ.

2025-02-04T11:12:27Z

ANTIBIOTIC RESISTANCE PATTERNS OF COMMON UROPATHOGENS ISOLATED FROM FEMALES AT ZAKHO CITY, KURDISTAN REGION, IRAQ. Adil ISSA, Fawzi Background: Urinary tract infection (UTI) is an infection in any part of the urinary system, including kidneys, ureters, bladder, and urethra. Uropathogenic bacterial-antibiotic resistance has become a severe challenge among UTI-causative agents. Objective: This work attempted to screen the activity of a wide range of antibiotics routinely used for UTI-derived infection management to assess the impact of antibiotics on some common UTI pathogens isolated from females. Method: This retrospective study was performed at Zakho Emergency Hospital, Kurdistan region of Iraq, from January 2016 to December 2019. Conventional bacteriological tests were used to identify the most common isolated uropathogens in females. The antibiotic sensitivity test was performed according to the Clinical and Laboratory Standards Institute (CLSI). The bacterial-antibiotics assay was determined using the disk diffusion (Kirby-Bauer) method, which depended on the microbiology laboratory records. Results: Out of 1730 urine samples, 1040 (60.4%) were found to be pathogens-positive samples. The most common uropathogens isolated were Staphylococcus spp 44% (n=460), Escherichia coli strains 25.35% (n=265), Klebsiella spp 15.78% (n=65), and Streptococcus spp 14.83 (n=155). Regarding the microbial-antibiotic resistance, ≤ 25% of the UTI investigated cases, except Streptococcus spp, meropenem, imipenem, and amikacin, showed a remarkable effect against all addressed pathogens. Vancomycin was the first choice against gram-positive bacteria in addition to rifampicin and doxycycline for Streptococcus spp. Gentamycin was found to be the most effective antimicrobial against Klebsiella spp. Concerning bacterial-antibiotic resistance ≥75% and excluding Streptococcus spp, amoxicillin, ampicillin/cloxacillin, erythromycin, clindamycin, cloxacillin, and metronidazole were completely non-functional against all bacteria. Azithromycin, norfloxacin, oxacillin, cefixime, nalidixic acid, and ceftazidime showed relatively weak activity against gram-positive bacteria in addition to cephalexin, ceftriaxone, and cloxacillin for Streptococcus spp. Ampicillin, augmentin, penicillin, and cephalexin were comparatively non-functional against gram-negative pathogens in addition to vancomycin, rifampicin, cephalothin, oxacillin, and trimethoprim for Klebsiella spp. All remaining antibiotics produced an activity ranging between ≥25% to ≤75% of examined cases. The results of this work may help clinicians to accurate their antibiotic-bacterial infection empirical treatment. Conclusion: All subjected bacteria exhibited a strong resistance to a broad spectrum of antibiotics. Therefore, except for imipenem, meropenem, or amikacin, an antibiotic sensitivity test should be conducted prior to prescribing any antibiotic.

A NEW CONJUGATE GRADIENT METHOD BASED ON LOGISTIC MAPPING FOR UNCONSTRAINED OPTIMIZATION AND ITS APPLICATION IN REGRESSION ANALYSIS

2025-02-04T11:12:38Z

A NEW CONJUGATE GRADIENT METHOD BASED ON LOGISTIC MAPPING FOR UNCONSTRAINED OPTIMIZATION AND ITS APPLICATION IN REGRESSION ANALYSIS Ahmad Hamad, Sarwar; Haji Omar, Dlovan; Abdulqader Sulaiman, Diman; Luqman Ibrahim, Alaa The study tackles the critical need for efficient optimization techniques in unconstrained optimization problems, where conventional techniques often suffer from slow and inefficient convergence. There is still a need for algorithms that strike a balance between computational efficiency and robustness, despite advancements in gradient-based techniques. This work introduces a novel conjugate gradient algorithm based on the logistic mapping formula. As part of the methodology, descent conditions are established, and the suggested algorithm's global convergence properties are thoroughly examined. Comprehensive numerical experiments are used for empirical validation, and the new algorithm is compared to the Polak-Ribière-Polyak (PRP) algorithm. The suggested approach performs better than the PR algorithm, according to the results, and is more efficient since it needs fewer function evaluations and iterations to reach convergence. Furthermore, the usefulness of the suggested approach is demonstrated by its actual use in regression analysis, notably in the modelling of population estimates for the Kurdistan Region of Iraq. In contrast to conventional least squares techniques, the method maintains low relative error rates while producing accurate predictions. All things considered, this study presents the novel conjugate gradient algorithm as an effective tool for handling challenging optimisation problems in both theoretical and real-world contexts.

MICROSCOPIC ARCHITECTURE OF THE RESPIRATORY AND CONDUCTING SYSTEM OF THE LUNG OF NILE MONITOR

2025-02-04T11:12:33Z

MICROSCOPIC ARCHITECTURE OF THE RESPIRATORY AND CONDUCTING SYSTEM OF THE LUNG OF NILE MONITOR Abd-Elhafeezd, Hanan H.; Al Sulivany, Basim S.A.; Salah, Abdullah S.; Ali, Mennatallah; Mohamed, Nor E.; Soliman, Soha A. Using semi-thin sections, the present investigation examined the microscopic characteristics of the lungs of the Nile monitor (Varanus niloticus). The lungs were composed of Intrapulmonary conducting airways and respiratory faveoli. Intrapulmonary airways originate from the terminal portion of the bronchus, which extends into the lung to create the bronchial tree. The bronchus was lined with pseudostratified ciliated epithelium composed of both ciliated and non-ciliated cells, and it was supported by plates of hyaline cartilage. The central lumen is surrounded by contractile fibers that contain smooth muscle cell bundles and are covered by ciliated and non-ciliated cells. The central lumen communicates with the faveoli. Separating adjacent faveoli are pulmonary trabeculae covered with various cell types, including type I pneumocytes, type II pneumocytes, and pulmonary macrophages. Some substantial pulmonary bronchi were also supported by small cartilage plate granules and lined with ciliated epithelium. Type I pneumocytes were flat cells, whereas type II pneumocytes had cuboidal cells with vacuolated cytoplasm. Surface irregularity and vacuolated cytoplasm were features of pulmonary macrophages. In addition, the connective tissue of the pulmonary septa contained immune cells, such as Mast and Eosinophils. In conclusion, the microstructure of the lung of the Nile monitor closely resembles that of other reptile species. However, the distinction between intrapulmonary cartilage palates and pulmonary septa raises the concept of species differentiation. In addition, the discovery of various types of pulmonary immune cells enhances the Nile monitor's ability to persist in a variety of environments by enhancing its pulmonary immunity.

ENHANCE THE AQUEOUS SOLUBILITY OF DICLOFENAC THROUGH THE SYNTHESIS OF DICLOFENAC-INOSITOL PRODRUG

2025-02-04T11:12:39Z

ENHANCE THE AQUEOUS SOLUBILITY OF DICLOFENAC THROUGH THE SYNTHESIS OF DICLOFENAC-INOSITOL PRODRUG Latif Qader, Hemn; Abdulrahman alsheikhly, Omar; Abdulla Hamadameen Hama, Hewa; Burhan Qader, Adnan Due to limited aqueous solubility, poor bioavailability makes oral dosage formulations difficult to formulate. Chemical alteration of medicinal compounds improves solubility. Prodrug design is a popular molecular modification method that improves solubility and oral bioavailability. This study aims to synthesize a diclofenac prodrug to enhance aqueous solubility. In this study, diclofenac was esterified with inositol to make a prodrug (DIP), which was identified by 1H-NMR and FT-IR. A computational pharmacokinetic software was used to study DIP's pharmacokinetic profile, and saturation solubility was measured in phosphate buffer (pH 6.8) and 0.1 HCl (pH 1.2) solutions. The ester bands of (C=O) stretch at 1739 cm-1 and the elimination of H signals of carboxylic acid at 10-12 ppm in the 1H-NMR spectrum proved the synthesis of (DIP). Diclofenac solubility increased 827-fold in phosphate buffer solution (p < 0.05) from 0.059± 0.0164 mg/ml to 48.8± 0.034 mg/ml, primarily due to polarity change. The solubility of diclofenac and (DIP) in 0.1 N HCl (pH 1.2) was 0.016± 0.0031 and 0.018± 0.002, respectively. The improvement in solubility in the acidic medium was non-significant (p > 0.05) due to acid hydrolysis of the ester bond between inositol and the drug. The synthesis of diclofenac prodrug can greatly enhance its water solubility.